Anti-Cd14 Antibody Picoband™

Product Name

Anti-Cd14 Antibody Picoband™

View all CD14 Antibodies

SKU/Catalog Number

A00137-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cd14 Antibody Picoband™ catalog # A00137-3. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Cd14 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00137-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived mouse Cd14 recombinant protein (Position: D36-A260). Mouse Cd14 shares 68.2% and 85.7% amino acid (aa) sequence identity with human and rat CD14, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00137-3 is reactive to CD14 in Mouse

Applications

A00137-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

40.076kDa

Background of CD14

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse
ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Cd14 using anti-Cd14 antibody (A00137-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd14 antigen affinity purified polyclonal antibody (Catalog # A00137-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd14 at approximately 50 kDa. The expected band size for Cd14 is at 39 kDa.

Gene/Protein Information For CD14 (Source: Uniprot.org, NCBI)

Alternative Names

CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein CD14 CD14 molecule monocyte differentiation antigen CD14|myeloid cell-specific leucine-rich glycoprotein

For more info on CD14, check out the CD14 Infographic

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In this infographic, you will see the following information for CD14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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